I can see 2 problems with storing at room temp...
Without nitrite, cure#1, any botulism bacteria the meat picked up will still be active....
Storing in an air tight bag, any "regular" bacteria can use up all the oxygen in the bag leaving a depleted oxygen atmosphere for the botulism bacteria to flourish...
Conditions for Germination of Spores in Food. Certain environmental conditions make it easier for spores to germinate and produce the botulinum toxin. These conditions include:
• Absence of oxygen, such as a covered storage pot, lidded jar, or other covered container, or a covering substance. For example, covering food with oil or grease (such as for grilled vegetables) can create the anaerobic environment in which spores can more easily germinate.
• A pH environment of 4.6 or higher (a low-acid environment). Note that a low pH (an acidic environment) does not destroy or inactivate toxins that have already been produced.
• A salt level lower than 7%. However, a high salt level may not prevent the bacterium from germinating in whole, salted foods, such as whole, uneviscerated, salted fish. Refer to the outbreak notes for salted fish.
• A sugar content lower than 50%.
• Warm temperatures, between 40 F and 120 F (4 C to 49 C), such as room temperature.
• A high moisture content.
• A lack of competing bacterial flora.
Nitrates, ascorbates, alcohol, refrigeration, and freezing can prevent Clostridium spores from germinating and producing toxins. However, nitrates, ascorbates, etc. do not destroy the toxins once the toxins have been produced.
Home-canned jams and jellies, because of their high sugar content, do not usually provide an environment in which the spores germinate. However, home-canned vegetables and meats do not usually have a high sugar content, and can become contaminated during the canning process.
These are the last of my first attempt at making brats! They were vacuum sealed and frozen, pulled them out of the freezer last night to cook on Sunday, but forgot to put them in the fridge before I went to bed. They were out for probably 10 hours, but were definitely room temperature this...
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