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Ok, thanks!

I appreciate the input but where I am I don't trust the local flora and fauna to give a good product. I am able to get lacto pickles going pretty easily with wild strains, but there is other stuff around. My house is prone to mildew (classic crappy Japanese rabbit hutch post war construction)...

Great! Thanks for sharing your knowledge! I will take it all in consideration and plan this out before I give it a try. Just clarifying I am not travelling with these. A friend brought a couple freeze dried packages from the US to me in Japan and I want to keep a stock of these cultures on...

Jim, thank you for the detailed response! I hope you dont mind my follow-up questions. For rapid freezing, I can get my hands on dry ice which can drop samples to -70C temporarily. Good enough? I might be able to get a McFarland reference but if I can't, would that be a big problem? Its a...

I am in Japan and don't have easy or cheap access to cultures. It requires having a friend who is travelling overseas to pick up and bring back in their suitcase. After the long wait for the cultures, I of course must repay the person with gifts etc so it ends up adding another cost...